EMD-20489 Experiments and Validation

Human alpha3beta4 nicotinic acetylcholine receptor in complex with nicotine in saposin nanodiscs without CHS

Single particle reconstruction
Overview of EMD-20489
Sample name: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to nicotine
Organism: Homo sapiens
Related EM entries by publication: EMD-20487, EMD-20488, EMD-20490

Map parameters

Recommended contour level: 0.013 (author)
Number of grid points: 300 × 300 × 300
Voxel size: 0.89 × 0.89 × 0.89 Å
Minimum density: -0.026
Maximum density: 0.036
Average density: 0.00
Standard deviation: 0.003

Sample information

Sample name: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to nicotine
Protein: Human alpha3beta4 nicotinic receptor in complex with Fab fragments and bound to nicotine

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 74820
Reconstruction protocol: BACK PROJECTION

Imaging

Session
Microscope model: FEI TALOS ARCTICA
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 64.0
Nominal CS (mm): 2.70
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Acquisition
Number of images: 1020

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 6.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100
Details: 4 second blot.