EMD-20463

Single-particle
3.0 Å
EMD-20463
3D View Gallery
Deposition: 13/07/2019
Map released: 25/03/2020
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20463
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6pst summary report (pdf.gz) Map and 6pst FULL report (pdf.gz)
EMICSS annotations (xml)

Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1.5b) with TraR and mutant rpsT P2 promoter

EMD-20463

Single-particle
3.0 Å
EMD-20463
3D View Gallery
Deposition: 13/07/2019
Map released: 25/03/2020
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 93666
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: INSILICO MODEL
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 256 256 256
Origin 0 0 0
Spacing 256 256 256
Voxel size 1.3 Å 1.3 Å 1.3 Å
Contour list
Primary Level Source
True 0.025 AUTHOR