EMD-20456 Function and Biology

Cryo-EM structure of HzTransib/TIR DNA transposon end complex (TEC)

Single particle reconstruction
4.2Å resolution
Overview of EMD-20456
Sample name: Transposon end complex of HzTransib with cleaved TIR substrate DNA
Number of unique components: 4

Transposon end complex of HzTransib with cleaved TIR substrate DNA [Helicoverpa zea]

Protein type
Name: Transposon end complex of HzTransib with cleaved TIR substrate DNA
Expression system
Expression system: Spodoptera frugiperda
Cell line: Sf9

Putative DNA-mediated transposase [Helicoverpa zea]

Protein type
Name: Putative DNA-mediated transposase
Theoretical weight (MDa): 0.057
Number of copies: 2
Expression system
Expression system: Spodoptera frugiperda

DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3') [Helicoverpa zea]

Nucleic-acid type
Name: DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3')
Theoretical weight (MDa): 0.005
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DC)(DA)(DC)(DG)(DG)(DT)(DG)(DG)(DA)(DT)(DC)(DG)(DA)(DA)(DA)(DA)

DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*CP*CP*AP*CP*CP*GP*TP*G)-3') [Helicoverpa zea]

Nucleic-acid type
Name: DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*CP*CP*AP*CP*CP*GP*TP*G)-3')
Theoretical weight (MDa): 0.005
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DT)(DT)(DT)(DT)(DC)(DG)(DA)(DT)(DC)(DC)(DA)(DC)(DC)(DG)(DT)(DG)