EMD-20452 Function and Biology

Cryo-EM structure of HzTransib/intact TIR substrate DNA pre-reaction complex (PRC)

Single particle reconstruction
3.4Å resolution
Overview of EMD-20452
Sample name: Pre-reaction complex of HzTransib with intact TIR substrate DNA
Number of unique components: 7

Pre-reaction complex of HzTransib with intact TIR substrate DNA [Helicoverpa zea]

Protein type
Name: Pre-reaction complex of HzTransib with intact TIR substrate DNA
Expression system
Expression system: Spodoptera frugiperda
Cell line: Sf9

DNA-mediated transposase [Helicoverpa zea]

Protein type
Name: DNA-mediated transposase
Theoretical weight (MDa): 0.057
Number of copies: 2
Expression system
Expression system: Spodoptera frugiperda

DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*TP*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3') [Helicoverpa zea]

Nucleic-acid type
Name: DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*TP*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3')
Theoretical weight (MDa): 0.007
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DC)(DT)(DA)(DG)(DA)(DT)(DC)(DT)(DC)(DA)(DC)(DG)(DG)(DT)(DG)(DG)(DA)(DT)(DC)(DG) (DA)(DA)(DA)(DA)

DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*CP*CP*AP*CP*CP*GP*TP*GP*AP*GP*AP*TP*CP*TP*AP*G)-3') [Helicoverpa zea]

Nucleic-acid type
Name: DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*CP*CP*AP*CP*CP*GP*TP*GP*AP*GP*AP*TP*CP*TP*AP*G)-3')
Theoretical weight (MDa): 0.007
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DT)(DT)(DT)(DT)(DC)(DG)(DA)(DT)(DC)(DC)(DA)(DC)(DC)(DG)(DT)(DG)(DA)(DG)(DA)(DT) (DC)(DT)(DA)(DG)

MAGNESIUM ION

Ligand type
Name: MAGNESIUM ION
Theoretical weight (MDa): 0
Number of copies: 2

ZINC ION

Ligand type
Name: ZINC ION
Theoretical weight (MDa): 0
Number of copies: 2

POTASSIUM ION

Ligand type
Name: POTASSIUM ION
Theoretical weight (MDa): 0
Number of copies: 2