EMD-20340

Subtomogram averaging
26.0 Å
EMD-20340
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Deposition: 25/06/2019
Map released: 06/11/2019
Last modified: 27/11/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20340
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

The Nexin-dynein Regulatory Complex (N-DRC) from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas drc11 axonemes

EMD-20340

Subtomogram averaging
26.0 Å
EMD-20340
3D View Gallery
Deposition: 25/06/2019
Map released: 06/11/2019
Last modified: 27/11/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Cell
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 1.5 e/Å2
Image processing [1]
Final reconstruction
Resolution: 26.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: BACK PROJECTION
⌯ Applied Symmetry
Point group: C1
⦩ Final angle assignment
Type: OTHER
Extraction
Number of images used: 2544
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: N-DRC structure from drc11 Chlamydomonas
⬡ Geometry
X Y Z
Dimensions 100 80 110
Origin -117 -48 -53
Spacing 100 80 110
Voxel size 5.189 Å 5.189 Å 5.189 Å
Contour list
Primary Level Source
True 137.0 AUTHOR