EMD-20284

Single-particle
3.0 Å
EMD-20284
3D View Gallery
Deposition: 12/06/2019
Map released: 12/02/2020
Last modified: 19/02/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20284
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6pb0 summary report (pdf.gz) Map and 6pb0 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 1 receptor in complex with Gs protein and Nb35

EMD-20284

Single-particle
3.0 Å
EMD-20284
3D View Gallery
Deposition: 12/06/2019
Map released: 12/02/2020
Last modified: 19/02/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Urocortin1-bound CRF1R in complex Gs and Nb35
Complex [1]
Name: Urocortin1-bound CRF1R in complex Gs and Nb35
Natural source [1]
Organism: Homo sapiens
Recombinant Expression [1]
Organism Strain Cell Plasmid
Spodoptera frugiperda - - -
Protein [6]
LEVO
Name: Corticotropin-releasing factor receptor 1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [3]
Biological process:
cell surface receptor signaling pathway

Cellular location:
membrane integral component of membrane
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 43 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Spodoptera aff. frugiperda 2 RZ-2014 - - -
LEVO
Name: Urocortin
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [1]


Cellular location:
extracellular region
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 4 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
synthetic construct - - -
LEVO
Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [3]

Molecular function:
G-protein beta/gamma-subunit complex binding GTPase activity GTP binding
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 43 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Spodoptera frugiperda - - -
LEVO
Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [25]
Biological process:
cellular response to prostaglandin E stimulus G protein-coupled receptor signaling pathway G protein-coupled acetylcholine receptor signaling pathway cellular response to catecholamine stimulus phospholipase C-activating G protein-coupled receptor signaling pathway retina development in camera-type eye sensory perception of taste signal transduction adenylate cyclase-activating dopamine receptor signaling pathway cell population proliferation Ras protein signal transduction
Molecular function:
signaling receptor complex adaptor activity protein-containing complex binding GTPase activity GTPase binding
Cellular location:
lysosomal membrane cytosol cytoplasm synapse plasma membrane membrane extracellular exosome photoreceptor disc membrane extracellular vesicle heterotrimeric G-protein complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 37 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Spodoptera frugiperda - - -
LEVO
Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [11]
Biological process:
fibroblast proliferation cell population proliferation cellular response to prostaglandin E stimulus cellular response to catecholamine stimulus adenylate cyclase-activating dopamine receptor signaling pathway G protein-coupled receptor signaling pathway
Molecular function:
G-protein beta-subunit binding
Cellular location:
membrane extracellular exosome plasma membrane heterotrimeric G-protein complex
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 7 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Spodoptera frugiperda - - -
LEVO
Name: Nanobody 35
Number of copies: 1
Natural source
Organism: synthetic construct
Molecular weight
Experimental Theoretical Method
- 15 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
Ligand [2]
Name: CHOLESTEROL
HET code: CLR
Number of copies: 5
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 386 Da -
Name: PALMITIC ACID
HET code: PLM
Number of copies: 7
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 256 Da -