EMD-20270 Experiments and Validation

Structure of the K. lactis CBF3 core

Single particle reconstruction
Overview of EMD-20270
Sample name: K. lactis CBF3 core
Organism: Kluyveromyces lactis
Fitted atomic model: 6p7v
Related EM entry by publication: EMD-20271, EMD-20272

Map parameters

Recommended contour level: 0.015 (author)
Number of grid points: 288 × 288 × 288
Voxel size: 1.06 × 1.06 × 1.06 Å
Minimum density: -0.024
Maximum density: 0.051
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: K. lactis CBF3 core
Proteins: K. lactis CBF3 core, Ctf13, Skp1, Cep3

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 130143

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 65.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen support: unspecified
Vitrification
Cryogen: ETHANE