EMD-20049

Single-particle
3.3 Å
EMD-20049
3D View Gallery
Deposition: 01/04/2019
Map released: 12/06/2019
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20049
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6og1 summary report (pdf.gz) Map and 6og1 FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, pre-state

EMD-20049

Single-particle
3.3 Å
EMD-20049
3D View Gallery
Deposition: 01/04/2019
Map released: 12/06/2019
Last modified: 20/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 2.6 µm
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 56.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.3 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 221083
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: INSILICO MODEL
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: EM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
⬡ Geometry
X Y Z
Dimensions 256 256 256
Origin 0 0 0
Spacing 256 256 256
Voxel size 1.032 Å 1.032 Å 1.032 Å
Contour list
Primary Level Source
True 0.0464 AUTHOR