EMD-20041 Experiments and Validation

Precursor ribosomal RNA processing complex, State 1.

Single particle reconstruction
Overview of EMD-20041
Sample name: Precursor Ribosomal RNA Processing Complex
Organisms: Chaetomium thermophilum, Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
Fitted atomic model: 6of3
Related EM entry by publication: EMD-20040, EMD-20042

Map parameters

Recommended contour level: 4.50 (author)
Number of grid points: 224 × 224 × 224
Voxel size: 1.08 × 1.08 × 1.08 Å
Minimum density: -0.709
Maximum density: 27.61
Average density: 0.005
Standard deviation: 0.657

Sample information

Sample name: Precursor Ribosomal RNA Processing Complex
Ligands: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, MAGNESIUM ION
Proteins: Precursor Ribosomal RNA Processing Complex, Ribonuclease, CLP1_P domain-containing protein

Validation information

Experimental information

 

Image processing

Number of particles: 74582

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 40.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: NITROGEN