EMD-1502

Single-particle
11.0 Å
EMD-1502
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Deposition: 06/04/2008
Map released: 31/03/2009
Last modified: 07/11/2012
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EMD-1502
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EMICSS annotations (xml)

Initial location of the RNA-dependent RNA polymerase in the bacteriophage phi6 procapsid determined by cryo-electron microscopy

EMD-1502

Single-particle
11.0 Å
EMD-1502
3D View Gallery
Deposition: 06/04/2008
Map released: 31/03/2009
Last modified: 07/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.5 mg/mL
Buffer
pH: 7.5
Details: 10mM sodium phosphate, 1mM MgCl2, 150 mM NaCl
Staining
Type: NEGATIVE
Details: None
Vitrification
Cryogen name: ETHANE
Instrument: REICHERT-JUNG PLUNGER
Details: Vitrification instrument: Reichert-Jung cryo-fixation unit
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 1.0 µm - 2.3 µm
Nominal magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry, Eucentric
Alignment procedure: LEGACY (Astigmatism: Corrected at 300000 times magnification, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 36
Average electron dose per image: 15 e/Å2
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 11.0 Å ( BY AUTHOR)
Resolution method: FSC 0.33 CUT-OFF
Number of images used: 1072
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
Bsoft, PFT3DR - -
CTF correction
Details:Each particle phase flipped
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Map of a procapsid without the P2 protein, containing only proteins P1, P4 and P7
Details: ::::EMDATABANK.org::::EMD-1502::::
⬡ Geometry
X Y Z
Dimensions 234 234 234
Origin -117 -117 -117
Spacing 234 234 234
Voxel size 2.54 Å 2.54 Å 2.54 Å
Contour list
Primary Level Source
True 2.25 -