EMD-1365

Single-particle
11.75 Å
EMD-1365
3D View Gallery
Deposition: 21/05/2007
Map released: 24/05/2007
Last modified: 07/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1365
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 1pn6 summary report (pdf.gz) Map and PDB model 1pn7 summary report (pdf.gz) Map and PDB model 1pn8 summary report (pdf.gz) Map and 1pn6 FULL report (pdf.gz) Map and 1pn7 FULL report (pdf.gz) Map and 1pn8 FULL report (pdf.gz)
EMICSS annotations (xml)

Locking and unlocking of ribosomal motions.

EMD-1365

Single-particle
11.75 Å
EMD-1365
3D View Gallery
Deposition: 21/05/2007
Map released: 24/05/2007
Last modified: 07/11/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Vitrification
Cryogen name: ETHANE
Instrument: OTHER
Details: Rapid-freezing in liquid ethane
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal magnification: 50000.0
Calibrated magnification: 49696.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Cryo transfer
Alignment procedure: LEGACY
Minimum tilt angle: 0
Maximum tilt angle: 0
Temperature
Average: 93 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 14 µm
Image processing [1]
Final reconstruction
Resolution: 11.75 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER, package - -
CTF correction
Details:CTF correctionn of 3D map
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM map of E.coli 70S ribosome
Details: ::::EMDATABANK.org::::EMD-1365::::
⬡ Geometry
X Y Z
Dimensions 130 130 130
Origin -65 -65 -65
Spacing 130 130 130
Voxel size 2.82 Å 2.82 Å 2.82 Å
Contour list
Primary Level Source
True 55.7 -