EMD-1363 Experiments and Validation

Locking and unlocking of ribosomal motions.

Single particle reconstruction
Overview of EMD-1363
Sample name: EF-G bound Release Complex in the presence of Puromycin and GDPNP
Organisms: Escherichia coli, synthetic construct
Fitted atomic models: 1pn6, 1pn7, 1pn8
Related EM entries by publication: EMD-1362, EMD-1364, EMD-1365, EMD-1366

Map parameters

Recommended contour level: 56.80
Number of grid points: 130 × 130 × 130
Voxel size: 2.82 × 2.82 × 2.82 Å
Minimum density: -116.942
Maximum density: 253.154
Average density: 2.691
Standard deviation: 25.799

Sample information

Sample name: EF-G bound Release Complex in the presence of Puromycin and GDPNP
Ligands: Puromycin, EF-G, GDPNP
Ribosome: Release Complex

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: SPIDER, package
Number of particles: 1
Reconstruction protocol: Back projection
CTF correction: CTF correctionn of 3D map

Imaging

Session
Microscope model: FEI TECNAI 20
Electron source: FIELD EMISSION GUN
Nominal magnification: 50000.0
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: ZEISS SCAI
Sampling interval (μm): 14.0

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: NONE
Cryogen: ETHANE
Details: Rapid-freezing in liquid ethane