EMD-12896 Function and Biology

Drs2p-Cdc50p in the E1-AMPPCP state with PI4P bound

Single particle reconstruction
3.8Å resolution
Overview of EMD-12896
Sample name: Binary complex of Drs2p-Cdc50p with the regulatory lipid PI4P
Number of unique components: 9

Binary complex of Drs2p-Cdc50p with the regulatory lipid PI4P [Saccharomyces cerevisiae]

Protein type
Name: Binary complex of Drs2p-Cdc50p with the regulatory lipid PI4P
Expression system
Expression system: Saccharomyces cerevisiae
Strain: W303-1A dpep4
Vector: pYeDP60

Drs2p [Saccharomyces cerevisiae]

Protein type
Name: Drs2p
Expression system
Expression system: Saccharomyces cerevisiae
Strain: W303-1A dpep4
Vector: pYeDP60

Cdc50p [Saccharomyces cerevisiae]

Protein type
Name: Cdc50p
Expression system
Expression system: Saccharomyces cerevisiae
Strain: W303-1A dpep4
Vector: pYeDP60

Probable phospholipid-transporting ATPase DRS2,Probable phospholipid-transporting ATPase DRS2 [Saccharomyces cerevisiae (strain ATCC 204508 / S288c)]

Protein type
Name: Probable phospholipid-transporting ATPase DRS2,Probable phospholipid-transporting ATPase DRS2
Theoretical weight (MDa): 0.164
Number of copies: 1
Expression system
Expression system: Saccharomyces cerevisiae

Cell division control protein 50 [Saccharomyces cerevisiae (strain ATCC 204508 / S288c)]

Protein type
Name: Cell division control protein 50
Theoretical weight (MDa): 0.047
Number of copies: 1
Expression system
Expression system: Saccharomyces cerevisiae

(2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate

Ligand type
Name: (2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
Theoretical weight (MDa): 0.001
Number of copies: 1

PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER

Ligand type
Name: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER
Theoretical weight (MDa): 0.001
Number of copies: 1

MAGNESIUM ION

Ligand type
Name: MAGNESIUM ION
Theoretical weight (MDa): 0
Number of copies: 1

water

Ligand type
Name: water
Theoretical weight (MDa): 0
Number of copies: 3