EMD-12175 Experiments and Validation

Low resolution reconstruction of whole human telomerase class 2

Single particle reconstruction
Overview of EMD-12175
Sample name: Whole human telomerase reconstruction class 2
Organism: Homo sapiens

Map parameters

Recommended contour level: 0.008 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.11 × 1.11 × 1.11 Å
Minimum density: -0.018
Maximum density: 0.045
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: Whole human telomerase reconstruction class 2
Protein: Whole human telomerase reconstruction class 2

Validation information

Experimental information

 

Image processing

Software: RELION
Number of particles: 484847

Fitting

Fitting protocol: rigid body

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 47.0
Nominal CS (mm): 2.70
Nominal magnification: 81000.0
Defocus max (nm): 3,500.00
Defocus min (nm): 1,200.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Acquisition
Number of images: 43639

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100