EMD-12150 › Experiments and Validation

Immature HIV-1 Gag partial hexamer with 1 Gag subunit missing, obtained by multi-reference classification

Subtomogram averaging
Overview of EMD-12150
Sample name: Human immunodeficiency virus 1
Organism: Human immunodeficiency virus 1
Related EM entries by publication: EMD-12147, EMD-12148, EMD-12149, EMD-12151, EMD-12152, EMD-12153

Map parameters

Recommended contour level: 1.78 (author)
Number of grid points: 128 × 128 × 128
Voxel size: 2.70 × 2.70 × 2.70 Å
Minimum density: -6.42
Maximum density: 6.834
Average density: 0.024
Standard deviation: 0.797

Sample information

Sample name: Human immunodeficiency virus 1
Virus: Human immunodeficiency virus 1

Validation information

Visual analysis

Experimental information

 

Image processing

Applied symmetry: C1
Software: TOM Toolbox
Number of subtomograms averaged: 13643
Reconstruction protocol: BACK PROJECTION

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 3.6499999999999999
Energy filter: GIF Quantum LS
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen support: 20 mA glow discharge current
Vitrification
Apparatus: FEI VITROBOT MARK II
Cryogen: ETHANE

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