EMD-12132

Subtomogram averaging
30.0 Å
EMD-12132
3D View Gallery
Deposition: 17/12/2020
Map released: 17/02/2021
Last modified: 14/04/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-12132
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

singlet microtubules from the mouse sperm endpiece

EMD-12132

Subtomogram averaging
30.0 Å
EMD-12132
3D View Gallery
Deposition: 17/12/2020
Map released: 17/02/2021
Last modified: 14/04/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Cell
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Model: Quantifoil
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE-PROPANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 1.7 e/Å2
Image processing [1]
Final reconstruction
Resolution: 30.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
PEET 1.13.0 -
⦩ Final angle assignment
Type: OTHER
Extraction
Number of images used: 1581
Software [1]
Name Version Details
PEET 1.13.0 -
CTF correction
Software [1]
Name Version Details
PEET 1.13.0 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Subtomogram average of singlet microtubules from the mouse sperm endpiece
⬡ Geometry
X Y Z
Dimensions 50 50 50
Origin 0 0 0
Spacing 50 50 50
Voxel size 7.08 Å 7.08 Å 7.08 Å
Contour list
Primary Level Source
True 5.5 AUTHOR