EMD-12053 Experiments and Validation

Drosophila melanogaster TRAPP C8, C12 and C13 region map from the TRAPPIII complex

Single particle reconstruction
Overview of EMD-12053
Sample name: TRAPPIII complex
Organism: Drosophila melanogaster
Fitted atomic model: 7b6e

Map parameters

Recommended contour level: 0.028 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.25 × 1.25 × 1.25 Å
Minimum density: -0.049
Maximum density: 0.103
Average density: -0.00
Standard deviation: 0.003

Sample information

Sample name: TRAPPIII complex
Proteins: TRAPPIII complex, FI18195p1

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 353400

Fitting

Overall B value: 302.39999999999998
Refinement space: REAL

Imaging

Session
Microscope model: TFS KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 30.0
Nominal CS (mm): 2.70
Nominal magnification: 75000.0
Defocus max (nm): 4,000.00
Defocus min (nm): 2,200.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Details: 32% of the images were acquiring tilted the stage 19 degrees.
Acquisition
Number of images: 3671
Details: 1190 from the whole dataset were collected with the stage tilted at 19 degrees

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.05
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE
Humidity: 100