EMD-11821 Experiments and Validation

Intermediate assembly of the Large subunit from Leishmania major mitochondrial ribosome

Single particle reconstruction
Overview of EMD-11821
Sample name: Leishmania mitochondrial ribosome
Organism: Leishmania tarentolae
Fitted atomic model: 7am2

Map parameters

Recommended contour level: 0.04 (author)
Number of grid points: 408 × 408 × 408
Voxel size: 1.20 × 1.20 × 1.20 Å
Minimum density: -0.12
Maximum density: 0.222
Average density: -0.00
Standard deviation: 0.013

Sample information

Sample name: Leishmania mitochondrial ribosome
Ligands: GUANOSINE-5'-TRIPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: Ribosomal RNA, R1, R2, R5
Proteins: Leishmania mitochondrial ribosome, Ribosomal protein L3-like protein, uL4m, RIBOSOMAL_L9 domain-containing protein, Putative ribosomal protein L11, 50S ribosomal protein L13-like protein, Ribosomal_L18e/L15P domain-containing protein, Putative 50S ribosomal protein L17, bL19m, bL20m, bL21m, uL22m, uL23m, uL24m, bL28m, uL29m, uL30m, bL32m, bL35m, mL41, mL94, TRUD domain-containing protein, UA, mL95, RNA uridylyltransferase, mL67, mL71, mL81, mL98, Putative ribosomal protein L14, mL86, mL87, mL53, mL63, mL68, mL80, mL74, L51_S25_CI-B8 domain-containing protein, mL42, mL52,mL52, mL93, mL75, mL85, mL100, mL49, mL89, Peptidyl-prolyl cis-trans isomerase, mL72, mL84, mL76, mL99, mL88, mL70, mL59/64, Peptidyl-prolyl cis-trans isomerase, LIM zinc-binding domain-containing protein, DNAj-like protein, SpoU_methylase domain-containing protein, Pseudouridylate synthase-like protein, Acyl carrier protein, L0R8F8, G domain-containing protein, GTPase Der, DEAD/DEAH box helicase-like protein, G domain-containing protein, mL78, U6, U1, U3, U4, U5, mL78, U2, U8

Validation information

Experimental information

 

Image processing

Number of particles: 59200

Imaging

Session
Microscope model: FEI TALOS ARCTICA
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE