EMD-11712 Experiments and Validation

HDAC-DC-nucleosome

Single particle reconstruction
Overview of EMD-11712
Sample name: HDAC-PC-Nuc
Organisms: Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Xenopus laevis, unidentified plasmid

Map parameters

Recommended contour level: 0.07 (author)
Number of grid points: 256 × 256 × 256
Voxel size: 1.89 × 1.89 × 1.89 Å
Minimum density: -0.206
Maximum density: 1.154
Average density: 0.001
Standard deviation: 0.035

Sample information

Sample name: HDAC-PC-Nuc
Proteins: HDAC-PC-Nuc, Histone deacetylase, Histone, DNA

Validation information

Experimental information

 

Image processing

Software: cisTEM
Number of particles: 9534

Fitting

Details: Real space refinement

Imaging

Session
Microscope model: TFS GLACIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.799999999999997
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE