EMD-11681

Single-particle
3.6 Å
EMD-11681
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Deposition: 01/09/2020
Map released: 16/09/2020
Last modified: 16/12/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11681
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7a91 summary report (pdf.gz) Map and 7a91 FULL report (pdf.gz)
EMICSS annotations (xml)

Dissociated S1 domain of SARS-CoV-2 Spike bound to ACE2 (Non-Uniform Refinement)

EMD-11681

Single-particle
3.6 Å
EMD-11681
3D View Gallery
Deposition: 01/09/2020
Map released: 16/09/2020
Last modified: 16/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Grid
Mesh: 200
Model: Quantifoil
Material: COPPER
Vitrification
Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 1.5 µm - 3.0 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Specialist optics
Energy filter
Name: GIF Quantum LS
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K2 QUANTUM (4k x 4k)
Detector mode: COUNTING
Average exposure time: 8.0 s
Average electron dose per image: 54.4 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 315000
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
CryoSPARC 2.15 -
Startup model [1]
Type: INSILICO MODEL
Insilico model: CryoSPARC Ab Initio
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2.15 -
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1.10 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 400 400 400
Origin 0 0 0
Spacing 400 400 400
Voxel size 1.078 Å 1.078 Å 1.078 Å
Contour list
Primary Level Source
True 0.5 AUTHOR