EMD-11667 Experiments and Validation

Nanodisc reconstituted human ABCB1 in complex with MRK16 Fab and vincristine

Single particle reconstruction
Overview of EMD-11667
Sample name: Nanodisc reconstituted human ABCB1 in complex with MRK16 Fab and vincristine
Organisms: Homo sapiens, Mus musculus
Fitted atomic model: 7a69
Related EM entries by publication: EMD-11666, EMD-11670, EMD-11671, EMD-11672

Map parameters

Recommended contour level: 0.008 (author)
Number of grid points: 384 × 384 × 384
Voxel size: 0.84 × 0.84 × 0.84 Å
Minimum density: -0.037
Maximum density: 0.067
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: Nanodisc reconstituted human ABCB1 in complex with MRK16 Fab and vincristine
Ligands: vincristine, CHOLESTEROL
Proteins: Nanodisc reconstituted human ABCB1 in complex with MRK16 Fab and vincristine, Multidrug resistance protein 1, MRK16 Fab-fragment light chain, MRK16 Fab-fragment heavy chain

Validation information

Experimental information

 

Image processing

Number of particles: 160170

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 32.0
Nominal CS (mm): 2.70
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.40
Vitrification
Cryogen: ETHANE