EMD-11657

Single-particle
1.7 Å
EMD-11657
3D View Gallery
Deposition: 22/08/2020
Map released: 18/11/2020
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11657
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7a5v summary report (pdf.gz) Map and 7a5v FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer, in complex with histamine and megabody Mb25 in lipid nanodisc

EMD-11657

Single-particle
1.7 Å
EMD-11657
3D View Gallery
Deposition: 22/08/2020
Map released: 18/11/2020
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.6
Grid
Mesh: 300
Model: UltrAuFoil R1.2/1.3
Material: GOLD
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 287 K
Instrument: LEICA PLUNGER
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.3 µm - 1.1 µm
Nominal magnification: 165000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: FEI FALCON IV (4k x 4k)
Dimensions: 4096 pixel x 4096 pixel
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 1.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 371693
Algorithm: FOURIER SPACE
⌯ Applied Symmetry
Point group: C5
Software [1]
Name Version Details
RELION 3.1 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.1 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.1 -
Final 3D classification
Software [1]
Name Version Details
RELION 3.1 -
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1.13 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 360 360 360
Origin 0 0 0
Spacing 360 360 360
Voxel size 0.8117 Å 0.8117 Å 0.8117 Å
Contour list
Primary Level Source
True 0.025 AUTHOR