EMD-11620

Single-particle
2.9 Å
EMD-11620
3D View Gallery
Deposition: 17/08/2020
Map released: 16/09/2020
Last modified: 14/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11620
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Class 3 of three-dimensional classification of single asymmetric units of RV-A89 into six classes. The class averages reveal differences at the RNA/protein interfaces.

EMD-11620

Single-particle
2.9 Å
EMD-11620
3D View Gallery
Deposition: 17/08/2020
Map released: 16/09/2020
Last modified: 14/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: TUNGSTEN HAIRPIN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average exposure time: 0.2 s
Average electron dose per image: 1.6 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 107054
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Class 3 of three-dimensional classification of single asymmetric units of RV-A89 into six classes. The class averages reveal differences at the RNA/protein interfaces.
⬡ Geometry
X Y Z
Dimensions 450 450 450
Origin 0 0 0
Spacing 450 450 450
Voxel size 0.97 Å 0.97 Å 0.97 Å
Contour list
Primary Level Source
True 0.002 AUTHOR