EMD-11594 Experiments and Validation

The structure of HAdV-D56 in complex with CD46

Single particle reconstruction
Overview of EMD-11594
Sample name: Human adenovirus 56
Organisms: Human adenovirus 56, Homo sapiens
Related EM entry by publication: EMD-11593

Map parameters

Recommended contour level: 0.004 (author)
Number of grid points: 1230 × 1230 × 1230
Voxel size: 1.04 × 1.04 × 1.04 Å
Minimum density: -0.007
Maximum density: 0.014
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: Human adenovirus 56
Protein: CD46 complement regulatory protein
Virus: Human adenovirus 56

Validation information

Experimental information

 

Image processing

Software: RELION
Number of particles: 1094

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 0.93999999999999995
Energy filter: GIF Bioquantum
Nominal magnification: 130000.0
Defocus max (nm): -3.00
Defocus min (nm): -0.80
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 3.60
Buffer: Commercial PBS buffer stock. Medicargo, PBS tablets, 09-9400-100
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 80