EMD-11236

Subtomogram averaging
11.4 Å
EMD-11236
3D View Gallery
Deposition: 29/06/2020
Map released: 14/10/2020
Last modified: 28/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11236
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6zjm summary report (pdf.gz) Map and 6zjm FULL report (pdf.gz)
EMICSS annotations (xml)

Reconstruction of Tula virus surface glycoprotein lattice

EMD-11236

Subtomogram averaging
11.4 Å
EMD-11236
3D View Gallery
Deposition: 29/06/2020
Map released: 14/10/2020
Last modified: 28/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.0
Vitrification
Cryogen name: ETHANE-PROPANE
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specialist optics
Energy filter
Name: GIF Quantum LS
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 4.71 e/Å2
Image processing [1]
Final reconstruction
Resolution: 11.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
⌯ Applied Symmetry
Point group: C4
Software [1]
Name Version Details
Dynamo - -
⦩ Final angle assignment
Type: OTHER
Details: Template matching
Software [1]
Name Version Details
Dynamo - -
Final 3D classification
Number of classes: 8
Software [1]
Name Version Details
RELION - -
Extraction
Number of images used: 107820
Reference model: EMD-4867
Method: Template matching
Software [1]
Name Version Details
Dynamo - -
CTF correction
Software [1]
Name Version Details
ETomo - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of Tula virus glycoprotein spike lattice.
⬡ Geometry
X Y Z
Dimensions 108 108 108
Origin 0 0 0
Spacing 108 108 108
Voxel size 4.0 Å 4.0 Å 4.0 Å
Contour list
Primary Level Source
True 1.5 AUTHOR