EMD-11170
126 helix bundle DNA nanostructure
EMD-11170
Single-particle9.8 Å
Deposition: 12/06/2020
Map released: 18/11/2020
Last modified: 30/12/2020
Details: Monodisperse, 1200nM
Buffer
pH: 8.0
Grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 293 K
Instrument: FEI VITROBOT MARK IV
Chamber humidity: 100%
Chamber temperature: 293 K
Instrument: FEI VITROBOT MARK IV
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 0.01 mm
Calibrated defocus: 0.3688 µm -
Nominal magnification: 47000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 0.01 mm
Calibrated defocus: 0.3688 µm -
Nominal magnification: 47000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
SPH aberration_corrector:
CEOS Cs corrector
Image Recording
[1]
Detector model:
FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Dimensions: 4096 pixel x 4096 pixel
Number of real images: 5005
Average electron dose per image: 50.0 e/Å2
Details: Magnified pixel size 1.39 A
Detector mode: INTEGRATING
Dimensions: 4096 pixel x 4096 pixel
Number of real images: 5005
Average electron dose per image: 50.0 e/Å2
Details: Magnified pixel size 1.39 A
Final
reconstruction
Resolution: 9.8
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 122493
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 122493
⌯ Applied Symmetry
Point group:
C1
⦨ Initial angle
assignment
⦩ Final angle assignment
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
CTFFIND | 4.1 | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Hollow cylinder shaped 126 helix bundle DNA nanostructure
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Hollow cylinder shaped 126 helix bundle DNA nanostructure
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 300 | 300 | 300 |
Origin | 0 | 0 | 0 |
Spacing | 300 | 300 | 300 |
Voxel size | 2.78 Å | 2.78 Å | 2.78 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.08 | AUTHOR |