EMD-11168

Single-particle
17.0 Å
EMD-11168
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Deposition: 12/06/2020
Map released: 18/11/2020
Last modified: 30/12/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11168
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Dumbbell_2

EMD-11168

Single-particle
17.0 Å
EMD-11168
3D View Gallery
Deposition: 12/06/2020
Map released: 18/11/2020
Last modified: 30/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Details: Monodisperse, 1000nM
Buffer
pH: 8.0
Grid
Mesh: 400
Model: C-flat-1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere: AIR
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 293 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 0.01 mm
Calibrated defocus: 1.292 µm - 5.141 µm
Nominal magnification: 18000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Details: Magnified pixel size 3.71 A
Specialist optics
SPH aberration_corrector: CEOS Cs corrector
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Dimensions: 4096 pixel x 4096 pixel
Number of real images: 736
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 17.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 18656
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 2 -
⦨ Initial angle assignment
Type: OTHER
Details: Maximum a posteriori optimisation (Relion -> REgularized LIkelihood OptimizatioN)
Software [1]
Name Version Details
RELION 2 -
⦩ Final angle assignment
Type: OTHER
Details: Maximum a posteriori optimisation (Relion -> REgularized LIkelihood OptimizatioN)
Software [1]
Name Version Details
RELION 2 -
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Dumbbell shaped DNA nanostructure with reduced helical twist density (by design) but without crossovers along the handle of the dumbbell
⬡ Geometry
X Y Z
Dimensions 300 300 300
Origin 0 0 0
Spacing 300 300 300
Voxel size 3.71 Å 3.71 Å 3.71 Å
Contour list
Primary Level Source
True 0.22 AUTHOR