EMD-11164

Helical reconstruction
3.5 Å
EMD-11164
3D View Gallery
Deposition: 11/06/2020
Map released: 17/02/2021
Last modified: 24/02/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11164
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6zch summary report (pdf.gz) Map and 6zch FULL report (pdf.gz)
EMICSS annotations (xml)

Amyloid fibril morphology II (ex vivo) from murine SAA1.1 protein.

EMD-11164

Helical reconstruction
3.5 Å
EMD-11164
3D View Gallery
Deposition: 11/06/2020
Map released: 17/02/2021
Last modified: 24/02/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Helical Array
Specimen preparation [1]
Buffer
pH: 7.0
Details: Water
Vitrification
Cryogen name: ETHANE
Chamber humidity: 96%
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 20.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.5 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 15505
⌯ Applied Symmetry
ΔΖ: 4.80846 Å
ΔΦ: -1.08807°
Axial symmetry: C1
Software [1]
Name Version Details
RELION 2.1 -
⦩ Final angle assignment
Type: NOT APPLICABLE
CTF correction
Software [1]
Name Version Details
Gctf - -
Segment selection [1]
Number selected Segment length Segment overlap Total filament length Details
15530 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Ex vivo mSAA amyloid fibril morphology II
⬡ Geometry
X Y Z
Dimensions 120 120 120
Origin 0 0 0
Spacing 120 120 120
Voxel size 1.35 Å 1.35 Å 1.35 Å
Contour list
Primary Level Source
True 13.0 AUTHOR