EMD-11089

Single-particle
4.1 Å
EMD-11089
3D View Gallery
Deposition: 28/05/2020
Map released: 13/01/2021
Last modified: 28/07/2021
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11089
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Transcription termination intermediate complex 3

EMD-11089

Single-particle
4.1 Å
EMD-11089
3D View Gallery
Deposition: 28/05/2020
Map released: 13/01/2021
Last modified: 28/07/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 5 mg/mL
Buffer
pH: 7.6
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 283 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 2.0 mm
Nominal defocus: 8.0 µm - 25.0 µm
Nominal magnification: 31000.0
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average exposure time: 10.0 s
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 28487
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
CryoSPARC 2.14 -
⦨ Initial angle assignment
Type: RANDOM ASSIGNMENT
Software [1]
Name Version Details
CryoSPARC 2.12 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2.14 -
Particle selection [1]
Selected Ref. model Method Software Details
2112819 - - - -
Final 3D classification
Software [1]
Name Version Details
CisTEM - -
CTF correction
Software [1]
Name Version Details
CTFFIND - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 300 300 300
Origin 0 0 0
Spacing 300 300 300
Voxel size 1.24 Å 1.24 Å 1.24 Å
Contour list
Primary Level Source
True 0.35 AUTHOR