EMD-11075
Tomogram of a influenza A/Hong Kong/1/1968 virions
EMD-11075
Tomography
Deposition: 26/05/2020Map released: 14/10/2020
Last modified: 02/12/2020
Buffer
pH: 7.5
Grid
Mesh: 200
Model: Quantifoil R2/2
Material: GOLD
Model: Quantifoil R2/2
Material: GOLD
Support Film [1]
| Material | Topology | Thickness |
|---|---|---|
| CARBON | - | - |
Vitrification
Fiducial Markers
| Manufacturer | Fiducial type | Diameter |
|---|---|---|
| BBI gold | - | 10 nanometer |
Sectioning
Details: NO SECTIONING
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 2.5 µm - 3.5 µm
Nominal magnification: 81000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 2.5 µm - 3.5 µm
Nominal magnification: 81000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
Energy filter
Image Recording
[1]
Detector model:
GATAN K2 QUANTUM (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 3.2 e/Å2
Detector mode: COUNTING
Average electron dose per image: 3.2 e/Å2
Final
reconstruction
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Tomogram showing a influenza A/Hong Kong/1968 virus
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Tomogram showing a influenza A/Hong Kong/1968 virus
⬡ Geometry
| X | Y | Z | |
|---|---|---|---|
| Dimensions | 960 | 928 | 250 |
| Origin | 0 | 0 | 0 |
| Spacing | 960 | 928 | 250 |
| Voxel size | 6.8 Å | 6.8 Å | 6.8 Å |
Contour list
| Primary | Level | Source |
|---|---|---|
| True | - | AUTHOR |