EMD-11063

Single-particle
2.97 Å
EMD-11063
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Deposition: 21/05/2020
Map released: 24/06/2020
Last modified: 07/10/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11063
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6z3r summary report (pdf.gz) Map and 6z3r FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of SMG1-8-9 kinase complex bound to UPF1-LSQ

EMD-11063

Single-particle
2.97 Å
EMD-11063
3D View Gallery
Deposition: 21/05/2020
Map released: 24/06/2020
Last modified: 07/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Mesh: 200
Model: Quantifoil R1.2/1.3
Material: COPPER
Vitrification
Cryogen name: ETHANE-PROPANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: OTHER
Imaging mode: OTHER
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Cooling holder cryogen: NITROGEN
Specialist optics
Energy filter
Name: GIF Quantum LS
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K3 (6k x 4k)
Number of grids: 1
Number of real images: 6293
Average exposure time: 5.5 s
Average electron dose per image: 68.75 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.97 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 481754
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3.0 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Particle selection [1]
Selected Ref. model Method Software Details
4368586 - - - -
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: SMG1-8-9 with bound UPF1-LSQ
⬡ Geometry
X Y Z
Dimensions 320 320 320
Origin 0 0 0
Spacing 320 320 320
Voxel size 1.096 Å 1.096 Å 1.096 Å
Contour list
Primary Level Source
True 0.0312 AUTHOR