EMD-10898

Single-particle
2.5 Å
EMD-10898
3D View Gallery
Deposition: 22/04/2020
Map released: 16/09/2020
Last modified: 14/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10898
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6ysi summary report (pdf.gz) Map and 6ysi FULL report (pdf.gz)
EMICSS annotations (xml)

Acinetobacter baumannii ribosome-tigecycline complex - 50S subunit

EMD-10898

Single-particle
2.5 Å
EMD-10898
3D View Gallery
Deposition: 22/04/2020
Map released: 16/09/2020
Last modified: 14/10/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 0.8 µm - 2.6 µm
Nominal magnification: 75000.0
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Average exposure time: 1.1 s
Average electron dose per image: 62.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.5 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 231159
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3.0 -
Startup model [1]
Type: INSILICO MODEL
Insilico model: Made by stochastic gradient descent in RELION 3.0
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
CTF correction
Software [1]
Name Version Details
Gctf 1.18 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Acinetobacter baumannii ribosome-tigecycline complex - 50S subunit, post-processed map
⬡ Geometry
X Y Z
Dimensions 400 400 400
Origin 0 0 0
Spacing 400 400 400
Voxel size 1.065 Å 1.065 Å 1.065 Å
Contour list
Primary Level Source
True 0.05 AUTHOR