EMD-10772

Single-particle
3.1 Å
EMD-10772 Deposition: 17/03/2020
Map released: 16/09/2020
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10772

Structure of a human 48S translational initiation complex - head

EMD-10772

Single-particle
3.1 Å
EMD-10772 Deposition: 17/03/2020
Map released: 16/09/2020
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Average exposure time: 1.0 s
Average electron dose per image: 107.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 144882
Startup model [1]
Type: PDB ENTRY
PDB model: 4KZY
Initial angle assignment
Type: OTHER
Final angle assignment
Type: OTHER
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Geometry
X Y Z
Dimensions 500 500 500
Origin 0 0 0
Spacing 500 500 500
Voxel size 1.074 Å 1.074 Å 1.074 Å
Contour list
Primary Level Source
True 0.02 AUTHOR