EMD-10726 Experiments and Validation

Electron cryo-tomography reconstruction and subvolume averaging of the A-C linker from the end of the proximal region of Paramecium tetraurelia basal body

Subtomogram averaging
Overview of EMD-10726
Sample name: Electron cryo-tomography reconstruction and subvolume averaging of the microtubule triplet from the start of the proximal region of Paramecium tetraurelia basal body
Organism: Paramecium tetraurelia
Related EM entries by publication: EMD-10727, EMD-10728, EMD-10729

Map parameters

Recommended contour level: 1.07 (author)
Number of grid points: 320 × 230 × 73
Voxel size: 3.45 × 3.45 × 3.45 Å
Minimum density: 0.411
Maximum density: 1.589
Average density: 0.877
Standard deviation: 0.167

Sample information

Sample name: Electron cryo-tomography reconstruction and subvolume averaging of the microtubule triplet from the start of the proximal region of Paramecium tetraurelia basal body
Cellular component: Electron cryo-tomography reconstruction and subvolume averaging of the microtubule triplet from the start of the proximal region of Paramecium tetraurelia basal body

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of subtomograms averaged: 899

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 100.0
Holder model: OTHER

Specimen

Preparation
Specimen state: cell
Vitrification
Cryogen: ETHANE