EMD-10577 Experiments and Validation

PKM2 in complex with Compound 6

Single particle reconstruction
Overview of EMD-10577
Sample name: Pyruvate Kinase M2
Organism: Homo sapiens
Fitted atomic model: 6tti
Related EM entries by publication: EMD-10563, EMD-10564, EMD-10574, EMD-10575, EMD-10576, EMD-10584

Map parameters

Recommended contour level: 0.07 (author)
Number of grid points: 167 × 227 × 267
Voxel size: 0.50 × 0.50 × 0.50 Å
Minimum density: -0.33
Maximum density: 0.529
Average density: 0.003
Standard deviation: 0.038

Sample information

Sample name: Pyruvate Kinase M2
Ligands: ~{N}-[3-(trifluoromethyl)-1,2-oxazol-5-yl]ethanamide, BETA-FRUCTOSE-1,6-DIPHOSPHATE, DIMETHYL SULFOXIDE, water
Proteins: Pyruvate Kinase M2, Pyruvate kinase PKM

Validation information

Experimental information

 

Image processing

Applied symmetry: D2
Software: RELION
Number of particles: 174554

Imaging

Session
Microscope model: OTHER
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 65.400000000000006
Holder model: OTHER
Acquisition
Number of images: 590

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.16
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100