EMD-10519

Single-particle
2.8 Å
EMD-10519
3D View Gallery
Deposition: 04/12/2019
Map released: 12/02/2020
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10519
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6tmf summary report (pdf.gz) Map and 6tmf FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of an archaeal ABCE1-bound ribosomal post-splitting complex

EMD-10519

Single-particle
2.8 Å
EMD-10519
3D View Gallery
Deposition: 04/12/2019
Map released: 12/02/2020
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 25.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 293010
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Composite map of post-processed maps
⬡ Geometry
X Y Z
Dimensions 360 360 360
Origin 0 0 0
Spacing 360 360 360
Voxel size 1.084 Å 1.084 Å 1.084 Å
Contour list
Primary Level Source
True 5.0 AUTHOR