EMD-10474
Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 with bound IF1
EMD-10474
Single-particle4.1 Å
Deposition: 10/11/2019
Map released: 27/11/2019
Last modified: 25/11/2020
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Details: 5 seconds blot.
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Details: 5 seconds blot.
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 2.7 mm
Nominal magnification: 130000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 2.7 mm
Nominal magnification: 130000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
Energy filter
Image Recording
[1]
Detector model:
GATAN K2 QUANTUM (4k x 4k)
Detector mode: COUNTING
Number of real images: 9045
Average exposure time: 10.0 s
Average electron dose per image: 36.3 e/Å2
Detector mode: COUNTING
Number of real images: 9045
Average exposure time: 10.0 s
Average electron dose per image: 36.3 e/Å2
Final
reconstruction
Resolution: 4.1
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 14310
Algorithm: FOURIER SPACE
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 14310
Algorithm: FOURIER SPACE
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | - | - |
⦨ Initial angle
assignment
Type:
MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Particle selection
[1]
Selected | Ref. model | Method | Software | Details |
---|---|---|---|---|
555269 | - | - | - | - |
Final 3D classification
Number of classes:
2
Software
[1]
Name | Version | Details |
---|---|---|
RELION | - | - |
CTF correction
Software
[1]
Name | Version | Details |
---|---|---|
RELION | - | - |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 440 | 440 | 440 |
Origin | 0 | 0 | 0 |
Spacing | 440 | 440 | 440 |
Voxel size | 1.05 Å | 1.05 Å | 1.05 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.015 | AUTHOR |