EMD-10473

Single-particle
3.92 Å
EMD-10473
3D View Gallery
Deposition: 10/11/2019
Map released: 27/11/2019
Last modified: 02/12/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10473
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6te0 summary report (pdf.gz) Map and 6te0 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3

EMD-10473

Single-particle
3.92 Å
EMD-10473
3D View Gallery
Deposition: 10/11/2019
Map released: 27/11/2019
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Details: 5 seconds blot.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 2.7 mm
Nominal magnification: 130000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
Energy filter
Name: GIF Quantum LS
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K2 QUANTUM (4k x 4k)
Detector mode: COUNTING
Number of real images: 9045
Average exposure time: 10.0 s
Average electron dose per image: 36.3 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.92 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 43232
Algorithm: FOURIER SPACE
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-3559
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
Particle selection [1]
Selected Ref. model Method Software Details
555269 - - - -
Final 3D classification
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
RELION - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Local resolution filtered map
⬡ Geometry
X Y Z
Dimensions 440 440 440
Origin 0 0 0
Spacing 440 440 440
Voxel size 1.05 Å 1.05 Å 1.05 Å
Contour list
Primary Level Source
True 0.017 AUTHOR