EMD-10417

Single-particle
3.4 Å
EMD-10417
3D View Gallery
Deposition: 28/10/2019
Map released: 18/11/2020
Last modified: 06/10/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10417
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

GroEL map obtained using the Preassis method for grid preparation

EMD-10417

Single-particle
3.4 Å
EMD-10417
3D View Gallery
Deposition: 28/10/2019
Map released: 18/11/2020
Last modified: 06/10/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Details: Specimen prepared using the novel freezing system Preassis
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 QUANTUM (4k x 4k)
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 59985
⌯ Applied Symmetry
Point group: D7
Startup model [1]
Type: OTHER
Details:Ab-initio SGD
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: GroEL map obtained using the Preassis method for sample preparation
⬡ Geometry
X Y Z
Dimensions 350 350 350
Origin 0 0 0
Spacing 350 350 350
Voxel size 0.83 Å 0.83 Å 0.83 Å
Contour list
Primary Level Source
True 0.0137 AUTHOR