EMD-10380

Single-particle
3.11 Å
EMD-10380
3D View Gallery
Deposition: 15/10/2019
Map released: 27/11/2019
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10380
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6t59 summary report (pdf.gz) Map and 6t59 FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex

EMD-10380

Single-particle
3.11 Å
EMD-10380
3D View Gallery
Deposition: 15/10/2019
Map released: 27/11/2019
Last modified: 02/12/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Average electron dose per image: 48.36 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.11 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 49626
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-10181
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of human TTC5 bound to the rabbit 80S ribosome translating beta-tubulin Sharpened, MTF-corrected map with the calibrated pixel size
⬡ Geometry
X Y Z
Dimensions 400 400 400
Origin 0 0 0
Spacing 400 400 400
Voxel size 1.33 Å 1.33 Å 1.33 Å
Contour list
Primary Level Source
True 0.08 AUTHOR