EMD-10291

Single-particle
4.5 Å
EMD-10291
3D View Gallery
Deposition: 05/09/2019
Map released: 06/11/2019
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10291
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structure of the Fanconi Anaemia core complex (focussed map for top region)

EMD-10291

Single-particle
4.5 Å
EMD-10291
3D View Gallery
Deposition: 05/09/2019
Map released: 06/11/2019
Last modified: 25/11/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.7 mg/mL
Buffer
pH: 8.0
Details: 50 mM HEPES pH 8.0, ~500 mM NaCl, 1 mM TCEP
Grid
Mesh: 300
Model: Quantifoil, UltrAuFoil, R1.2/1.3
Material: GOLD
Pretreatment
Type: PLASMA CLEANING
Atmosphere:
Support Film [1]
Material Topology Thickness
- HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277.15 K
Instrument: FEI VITROBOT MARK IV
Details: Blot time: 3 to 4.5 s Wait time: 0 s Drain time: 0 s Force: -10 N.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 2.7 mm
Nominal defocus: -1.8 µm - -4.0 µm
Nominal magnification: 75000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Number of real images: 4145
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.5 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 169000
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3.0 -
Startup model [1]
Type: OTHER
Details:Initial model generated in EMAN2.
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X Y Z
Dimensions 380 380 380
Origin -190 -190 -190
Spacing 380 380 380
Voxel size 1.07 Å 1.07 Å 1.07 Å
Contour list
Primary Level Source
True 0.0188 AUTHOR