EMD-10200

Single-particle
3.1 Å
EMD-10200
3D View Gallery
Deposition: 07/08/2019
Map released: 12/02/2020
Last modified: 30/09/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10200
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6shl summary report (pdf.gz) Map and 6shl FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of a marine algae virus of the order Picornavirales

EMD-10200

Single-particle
3.1 Å
EMD-10200
3D View Gallery
Deposition: 07/08/2019
Map released: 12/02/2020
Last modified: 30/09/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Mesh: 300
Model: Quantifoil R2/2
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 1.0 µm - 3.0 µm
Nominal magnification: 140000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Number of grids: 1
Number of real images: 2592
Average exposure time: 8.0 s
Average electron dose per image: 29.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 8315
Algorithm: FOURIER SPACE
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
RELION 2.1 -
⦨ Initial angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
RELION 2.1 -
⦩ Final angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
RELION 2.1 -
Particle selection [1]
Selected Ref. model Method Software Details
9920 - - - -
CTF correction
Software [1]
Name Version Details
CTFFIND 4 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 450 450 450
Origin 0 0 0
Spacing 450 450 450
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.127 AUTHOR