EMD-10152 Experiments and Validation

Class2 : CENP-A nucleosome in complex with CENP-C central region

Single particle reconstruction
Overview of EMD-10152
Sample name: CENP-A nucleosome
Organisms: Homo sapiens, synthetic construct
Fitted atomic model: 6se6

Map parameters

Recommended contour level: 0.013 (author)
Number of grid points: 336 × 336 × 336
Voxel size: 0.65 × 0.65 × 0.65 Å
Minimum density: -0.052
Maximum density: 0.111
Average density: 0.00
Standard deviation: 0.004

Sample information

Sample name: CENP-A nucleosome
Nucleic acids: DNA (145-MER), DNA (145-MER)
Proteins: CENP-A nucleosome, Histones, DNA, Histone H3-like centromeric protein A, Histone H4, Histone H2A type 2-A, Histone H2B type 1-C/E/F/G/I, Centromere protein C

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 320000

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 100.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 1.20
Vitrification
Cryogen: ETHANE