EMD-10105 Experiments and Validation

Structure of Azospirillum brasilense Glutamate Synthase in a4b3 oligomeric state

Single particle reconstruction
Overview of EMD-10105
Sample name: Glutamate Synthase complex in a4b3 oligomeric state
Organism: Azospirillum brasilense
Fitted atomic model: 6s6t
Related EM entries by publication: EMD-10104, EMD-10106, EMD-10108

Map parameters

Recommended contour level: 0.09 (author)
Number of grid points: 308 × 308 × 308
Voxel size: 1.43 × 1.43 × 1.43 Å
Minimum density: -0.071
Maximum density: 0.264
Average density: 0.00
Standard deviation: 0.01

Sample information

Sample name: Glutamate Synthase complex in a4b3 oligomeric state
Ligands: FLAVIN MONONUCLEOTIDE, FE3-S4 CLUSTER, IRON/SULFUR CLUSTER, FLAVIN-ADENINE DINUCLEOTIDE
Proteins: Glutamate Synthase complex in a4b3 oligomeric state, Glutamate synthase [NADPH] large chain, Glutamate synthase [NADPH] small chain

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 47735

Imaging

Session
Microscope model: FEI TALOS ARCTICA
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 3.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100