EMD-10104 Experiments and Validation

Structure of Azospirillum brasilense Glutamate Synthase in a4b4 oligomeric state.

Single particle reconstruction
Overview of EMD-10104
Sample name: Glutamate Synthase complex in a4b4 oligomeric state
Organism: Azospirillum brasilense
Fitted atomic model: 6s6s
Related EM entries by publication: EMD-10105, EMD-10106, EMD-10108

Map parameters

Recommended contour level: 0.09 (author)
Number of grid points: 308 × 308 × 308
Voxel size: 1.43 × 1.43 × 1.43 Å
Minimum density: 0.00
Maximum density: 1.00
Average density: 0.019
Standard deviation: 0.124

Sample information

Sample name: Glutamate Synthase complex in a4b4 oligomeric state
Ligands: FLAVIN MONONUCLEOTIDE, FE3-S4 CLUSTER, IRON/SULFUR CLUSTER, FLAVIN-ADENINE DINUCLEOTIDE
Proteins: Glutamate Synthase complex in a4b4 oligomeric state, Glutamate synthase [NADPH] large chain, Glutamate synthase [NADPH] small chain

Validation information

Experimental information

 

Image processing

Software: RELION
Number of particles: 54434

Imaging

Session
Microscope model: FEI TALOS ARCTICA
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 3.00
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100