EMD-10073 Experiments and Validation

Ribosome nascent chain in complex with SecA

Single particle reconstruction
Overview of EMD-10073
Sample name: ribosome nascent chain in complex with SecA
Organism: Escherichia coli
Fitted atomic model: 6s0k
Related EM entry by publication: EMD-10074

Map parameters

Recommended contour level: 0.82 (author)
Number of grid points: 320 × 320 × 320
Voxel size: 1.39 × 1.39 × 1.39 Å
Minimum density: -2.568
Maximum density: 6.126
Average density: 0.015
Standard deviation: 0.411

Sample information

Sample name: ribosome nascent chain in complex with SecA
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: tRNA-CCA, 23S ribosomal RNA, 5S ribosomal RNA
Proteins: ribosome nascent chain in complex with SecA, ribosome, SecA, nascent chain, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, Protein translocase subunit SecA, Cytoskeleton protein RodZ

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 37334

Fitting

Initial atomic model: 5GAG
Fitting protocol: rigid body

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 40.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: OTHER
Humidity: 95