EMD-10054 Function and Biology

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state C

Single particle reconstruction
7.1Å resolution
Overview of EMD-10054
Sample name: 90S pre-ribosome, state C
Number of unique components: 65

90S pre-ribosome, state C [Chaetomium thermophilum]

Protein type
Name: 90S pre-ribosome, state C

Periodic tryptophan protein 2-like protein [Chaetomium thermophilum]

Protein type
Name: Periodic tryptophan protein 2-like protein
Theoretical weight (MDa): 0.101
Number of copies: 1

Utp3 [Chaetomium thermophilum]

Protein type
Name: Utp3
Theoretical weight (MDa): 0.073
Number of copies: 1

Utp6 [Chaetomium thermophilum]

Protein type
Name: Utp6
Theoretical weight (MDa): 0.047
Number of copies: 1

Utp7 [Chaetomium thermophilum]

Protein type
Name: Utp7
Theoretical weight (MDa): 0.064
Number of copies: 1

U3 small nucleolar RNA-associated protein 11 [Chaetomium thermophilum]

Protein type
Name: U3 small nucleolar RNA-associated protein 11
Theoretical weight (MDa): 0.031
Number of copies: 1

Utp12 [Chaetomium thermophilum]

Protein type
Name: Utp12
Theoretical weight (MDa): 0.108
Number of copies: 1

Utp13 [Chaetomium thermophilum]

Protein type
Name: Utp13
Theoretical weight (MDa): 0.1
Number of copies: 1

Utp14 [Chaetomium thermophilum]

Protein type
Name: Utp14
Theoretical weight (MDa): 0.105
Number of copies: 1

Utp18 [Chaetomium thermophilum]

Protein type
Name: Utp18
Theoretical weight (MDa): 0.069
Number of copies: 1

Putative U3 snoRNP protein [Chaetomium thermophilum]

Protein type
Name: Putative U3 snoRNP protein
Theoretical weight (MDa): 0.115
Number of copies: 1

Utp24 [Chaetomium thermophilum]

Protein type
Name: Utp24
Theoretical weight (MDa): 0.022
Number of copies: 1

Imp3 [Chaetomium thermophilum]

Protein type
Name: Imp3
Theoretical weight (MDa): 0.022
Number of copies: 1

Putative U3 small nucleolar ribonucleoprotein [Chaetomium thermophilum]

Protein type
Name: Putative U3 small nucleolar ribonucleoprotein
Theoretical weight (MDa): 0.034
Number of copies: 1

Putative U3 small nucleolar ribonucleoprotein protein [Chaetomium thermophilum]

Protein type
Name: Putative U3 small nucleolar ribonucleoprotein protein
Theoretical weight (MDa): 0.086
Number of copies: 1

Sof1 [Chaetomium thermophilum]

Protein type
Name: Sof1
Theoretical weight (MDa): 0.051
Number of copies: 1

Emg1 [Chaetomium thermophilum]

Protein type
Name: Emg1
Theoretical weight (MDa): 0.028
Number of copies: 2

KRR1 small subunit processome component [Chaetomium thermophilum]

Protein type
Name: KRR1 small subunit processome component
Theoretical weight (MDa): 0.038
Number of copies: 1

Pre-rRNA-processing protein PNO1 [Chaetomium thermophilum]

Protein type
Name: Pre-rRNA-processing protein PNO1
Theoretical weight (MDa): 0.029
Number of copies: 1

40S ribosomal protein S1 [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S1
Theoretical weight (MDa): 0.029
Number of copies: 1

40S ribosomal protein s5-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein s5-like protein
Theoretical weight (MDa): 0.024
Number of copies: 1

40S ribosomal protein S7 [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S7
Theoretical weight (MDa): 0.023
Number of copies: 1

40S ribosomal protein s9-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein s9-like protein
Theoretical weight (MDa): 0.022
Number of copies: 1

40S ribosomal protein S13-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S13-like protein
Theoretical weight (MDa): 0.017
Number of copies: 1

40S ribosomal protein S14-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S14-like protein
Theoretical weight (MDa): 0.016
Number of copies: 1

40S ribosomal protein S16-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S16-like protein
Theoretical weight (MDa): 0.016
Number of copies: 1

40S ribosomal protein S22-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S22-like protein
Theoretical weight (MDa): 0.015
Number of copies: 1

40S ribosomal protein s23-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein s23-like protein
Theoretical weight (MDa): 0.016
Number of copies: 1

40S ribosomal protein S28-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S28-like protein
Theoretical weight (MDa): 0.008
Number of copies: 1

Ribosomal protein s27-like protein [Chaetomium thermophilum]

Protein type
Name: Ribosomal protein s27-like protein
Theoretical weight (MDa): 0.009
Number of copies: 1

Faf1 [Chaetomium thermophilum]

Protein type
Name: Faf1
Theoretical weight (MDa): 0.035
Number of copies: 1

35S ribosomal RNA [Chaetomium thermophilum]

Nucleic-acid type
Name: 35S ribosomal RNA
Theoretical weight (MDa): 0.599
Type: RNA
Structure: OTHER
Synthetic: false
Sequence: GGGGGGAUGUCCGGGUCGCCCUGGGCACCUCUCCCGGCAGCCUUCGCGGGCAGCCGUAGUCCCGGUUCAUACGGGCUCUG UGGGUGGGGGAAUUCAGGAGGGGAAAGCUUAGGCGCACGAUAGUUACCUGGUUGAUUCUGCCAGUAGUCAUAUGCUUGUC UCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUAUACAGCGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUU UAUUUGAUAGUACCUUACUACAUGGAUAACCGUGGUAAUUCUAGAGCUAAUACAUGCUGAAAAUCCCGACUUCGGAAGGG AUGUAUUUAUUAGAUUAAAAACCAAUGCCCUUCGGGGCUUUCUGGUGAUUCAUAAUAACUUCUCGAAUCGCACGGCCUUG CGCCGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGACGGCUGGGUAUUGGCCAGCCGUGGUGACAACGGGUA ACGGAGGGUUAGGGCUCGACCCCGGAGAAGGAGCCUGAGAAACGGCUACUACAUCCAAGGAAGGCAGCAGGCGCGCAAAU UACCCAAUCCCGACACGGGGAGGUAGUGACAAUAAAUACUGAUACAGGGCUCUUUCGGGUCUUGUAAUUGGAAUGAGUAC AAUUUAAAUCCCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUA UAUUAAAGUUGUUGAGGUUAAAAAGCUCGUAGUUGAACCUUGGGCCUAGCCCGCCGGUCCGCCUCACCGCGUGCACUGGC UGGGCUGGGCCUUUCCUUCUGGAGAACCGCAUGCCCUUCACUGGGUGUGUCGGGGAACCAGGACUUUUACUCUGAACAAA UUAGAUCGCUUAAAGAAGGCCUAUGCUUGAAUACAUUAGCAUGGAAUAAUAGAAUAGGACGUGUGGUUCUAUUUUGUUGG UUUCUAGGACCGCCGUAAUGAUUAAUAGGGACAGUCGGGGGCGUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUU AUUGAAGACUAACAACUGCGAAAGCAUUCGCCAAGGAUGUUUUCAUUAAUCAGGAACGAAAGUUAGGGGAUCGAAGACGA UCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGAUUAGGGAUCGGACGGCGUUUAUUUUUGACCCGUUCGGCACCU UACGAUAAAUCAAAAUGUUUGGGCUCCUGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGAAAUUGACGGAAGGGCACC ACCAGGGGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACGAUGAGGAUUGACAGAU UGAGAGCUCUUUCUUGAUUUCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGA UAACGAACGAGACCUUAACCUGCUAAAUAGCCCGUAUUGCCUUGGCAGUACGCCGGCUUCUUAGAGGGACUAUCGGCUCA AGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUUCUGGGCCGCACGCGCGCUACACUGACAGAG CCAGCGAGUACUCCCUUGGCCGAGAGGCCCGGGUAAUCUUGUUAAACUCUGUCGUGCUGGGGAUAGAGCAUUGCAAUUAU UGCUCUUCAACGAGGAAUCCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCC CGUCGCUACUACCGAUCGGAAAGUUAUCCAAACUCGGUCGUUUAGAGGAAGUAAAAGUCGUAACAAGGUCUCCGUAGGUG AACCUGCGGAGGGAUCAUU

U3 small nucleolar RNA-associated protein 22 [Chaetomium thermophilum]

Protein type
Name: U3 small nucleolar RNA-associated protein 22
Theoretical weight (MDa): 0.131
Number of copies: 1

Rrp7 [Chaetomium thermophilum]

Protein type
Name: Rrp7
Theoretical weight (MDa): 0.036
Number of copies: 1

Putative ribosomal protein [Chaetomium thermophilum]

Protein type
Name: Putative ribosomal protein
Theoretical weight (MDa): 0.018
Number of copies: 1

RNA 3'-terminal phosphate cyclase-like protein [Chaetomium thermophilum]

Protein type
Name: RNA 3'-terminal phosphate cyclase-like protein
Theoretical weight (MDa): 0.043
Number of copies: 1

Bms1 [Chaetomium thermophilum]

Protein type
Name: Bms1
Theoretical weight (MDa): 0.134
Number of copies: 1

Kre33 [Chaetomium thermophilum]

Protein type
Name: Kre33
Theoretical weight (MDa): 0.12
Number of copies: 2

40S ribosomal protein S4 [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S4
Theoretical weight (MDa): 0.03
Number of copies: 1

40S ribosomal protein S6 [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S6
Theoretical weight (MDa): 0.027
Number of copies: 1

40S ribosomal protein S8 [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S8
Theoretical weight (MDa): 0.023
Number of copies: 1

40S ribosomal protein S11-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S11-like protein
Theoretical weight (MDa): 0.019
Number of copies: 1

40S ribosomal protein S24 [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S24
Theoretical weight (MDa): 0.016
Number of copies: 1

Ribosome biogenesis protein ENP2 [Chaetomium thermophilum]

Protein type
Name: Ribosome biogenesis protein ENP2
Theoretical weight (MDa): 0.076
Number of copies: 1

Utp20 [Chaetomium thermophilum]

Protein type
Name: Utp20
Theoretical weight (MDa): 0.295
Number of copies: 1

Bfr2 [Chaetomium thermophilum]

Protein type
Name: Bfr2
Theoretical weight (MDa): 0.069
Number of copies: 1

Utp2 [Chaetomium thermophilum]

Protein type
Name: Utp2
Theoretical weight (MDa): 0.104
Number of copies: 1

Utp4 [Chaetomium thermophilum]

Protein type
Name: Utp4
Theoretical weight (MDa): 0.098
Number of copies: 1

UTP10 [Chaetomium thermophilum]

Protein type
Name: UTP10
Theoretical weight (MDa): 0.199
Number of copies: 1

Utp15 [Chaetomium thermophilum]

Protein type
Name: Utp15
Theoretical weight (MDa): 0.061
Number of copies: 1

Utp17 [Chaetomium thermophilum]

Protein type
Name: Utp17
Theoretical weight (MDa): 0.105
Number of copies: 1

Enp1 [Chaetomium thermophilum]

Protein type
Name: Enp1
Theoretical weight (MDa): 0.054
Number of copies: 1

Utp5 [Chaetomium thermophilum]

Protein type
Name: Utp5
Theoretical weight (MDa): 0.045
Number of copies: 2

Utp16 [Chaetomium thermophilum]

Protein type
Name: Utp16
Theoretical weight (MDa): 0.041
Number of copies: 1

Utp8 [Chaetomium thermophilum]

Protein type
Name: Utp8
Theoretical weight (MDa): 0.102
Number of copies: 1

Noc4 [Chaetomium thermophilum]

Protein type
Name: Noc4
Theoretical weight (MDa): 0.063
Number of copies: 1

Nop1 [Chaetomium thermophilum]

Protein type
Name: Nop1
Theoretical weight (MDa): 0.033
Number of copies: 2

Putative nucleolar protein [Chaetomium thermophilum]

Protein type
Name: Putative nucleolar protein
Theoretical weight (MDa): 0.058
Number of copies: 1

Nop58 [Chaetomium thermophilum]

Protein type
Name: Nop58
Theoretical weight (MDa): 0.064
Number of copies: 1

Snu13 [Chaetomium thermophilum]

Protein type
Name: Snu13
Theoretical weight (MDa): 0.014
Number of copies: 2

Rrp9 [Chaetomium thermophilum]

Protein type
Name: Rrp9
Theoretical weight (MDa): 0.07
Number of copies: 1

Fcf2 [Chaetomium thermophilum]

Protein type
Name: Fcf2
Theoretical weight (MDa): 0.023
Number of copies: 1

U3 snoRNA [Chaetomium thermophilum]

Nucleic-acid type
Name: U3 snoRNA
Theoretical weight (MDa): 0.075
Type: RNA
Structure: OTHER
Synthetic: false
Sequence: AGCGACAAUACUUCAGAGAAUCAUUUCUAUAGUAGUUGUCCUCUCUUGUGUUUCCUAAAGGAGCCACAGAUCCCACCCGG GUUGAUGAACGAGAUCCUCGGCGCCAGUGAGGUCCAUUUUUACUCUCGCUCUACCUGCAAAGGUGGCGGUCGCGUGCCUC GUCUCGCGGCUGUAUAGAGAGUGGCGAUGAUCUGUACCCCGCGGGGUGGGUGUCGAUGGAAGUCUGACCGGC

40S ribosomal protein S19-like protein [Chaetomium thermophilum]

Protein type
Name: 40S ribosomal protein S19-like protein
Theoretical weight (MDa): 0.017
Number of copies: 1

ZINC ION

Ligand type
Name: ZINC ION
Theoretical weight (MDa): 0
Number of copies: 2