EMD-0798 Experiments and Validation

F-actin part of cardiac thin filament in low calcium state, actin levels VI-VII, I

Single particle reconstruction
Overview of EMD-0798
Sample name: F-actin of cardiac thin filament
Organism: Mus musculus

Map parameters

Recommended contour level: 0.023 (author)
Number of grid points: 264 × 264 × 264
Voxel size: 1.07 × 1.07 × 1.07 Å
Minimum density: -0.012
Maximum density: 0.069
Average density: 0.002
Standard deviation: 0.004

Sample information

Sample name: F-actin of cardiac thin filament
Cellular component: F-actin of cardiac thin filament

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 450179

Fitting

Initial atomic model: 4Y99

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Energy filter: GIF Quantum LS
Nominal magnification: 81000.0
Defocus max (nm): 1,100.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: filament
Specimen concentration (mg/mL): 0.10
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100