EMD-0797

Single-particle
5.3 Å
EMD-0797
3D View Gallery
Deposition: 30/09/2019
Map released: 15/01/2020
Last modified: 11/03/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0797
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Tropomyosin in low calcium state, actin levels II-IV

EMD-0797

Single-particle
5.3 Å
EMD-0797
3D View Gallery
Deposition: 30/09/2019
Map released: 15/01/2020
Last modified: 11/03/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Filament
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 7.2
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277.15 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal magnification: 81000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Specialist optics
Phase plate: VOLTA PHASE PLATE
Energy filter
Name: GIF Quantum LS
Slit width: 20 eV
Image Recording [1]
Detector model: GATAN K3 (6k x 4k)
Average exposure time: 5.6 s
Average electron dose per image: 60.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 5.3 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 471020
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3.0.6 -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-7938
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
RELION 3.0.6 -
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
RELION 3.0.6 -
CTF correction
Software [1]
Name Version Details
Gctf 1.18 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Tropomyosin in low calcium state, actin levels II-IV
⬡ Geometry
X Y Z
Dimensions 264 264 264
Origin 0 0 0
Spacing 264 264 264
Voxel size 1.07 Å 1.07 Å 1.07 Å
Contour list
Primary Level Source
True 0.019 AUTHOR