EMD-0772

Single-particle
15.7 Å
EMD-0772
3D View Gallery
Deposition: 24/08/2019
Map released: 04/12/2019
Last modified: 04/12/2019
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0772
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

H3-H3-H3 tri-nucleosome with the 30 base-pair linker DNA

EMD-0772

Single-particle
15.7 Å
EMD-0772
3D View Gallery
Deposition: 24/08/2019
Map released: 04/12/2019
Last modified: 04/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.03 mg/mL
Buffer
pH: 7.8
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TALOS ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: INTEGRATING
Number of grids: 1
Number of real images: 8035
Average exposure time: 1.0 s
Average electron dose per image: 25.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 15.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 4534
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: OTHER
Details:Cylinder generated by SPIDER
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
Particle selection [1]
Selected Ref. model Method Software Details
109525 - - - -
Final 3D classification
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
CTFFIND - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: H3-H3-H3 tri-nucleosome with the 30 base-pair linker DNA
⬡ Geometry
X Y Z
Dimensions 280 280 280
Origin 0 0 0
Spacing 280 280 280
Voxel size 1.4 Å 1.4 Å 1.4 Å
Contour list
Primary Level Source
True 3.6 AUTHOR